Analytical methods for quantification of vitamin D and implications for diagnosis of vitamin D deficiency among children and adolescents

Abstract

Accurate measurement of vitamin D is essential for diagnosing and managing vitamin D deficiency in both children, adolescents, and the general population. This study evaluates analytical methods used by medical laboratories and estimates hypovitaminosis D prevalence in these groups. Two surveys were conducted: one with 16 laboratories assessing assay methods, quality controls, and hypovitaminosis frequency, the other with 30 doctors providing clinical insight. Results show varied practices, mostly using immunoassays, with differences in quality control. Hypovitaminosis D was frequent in both populations. The study emphasizes the need to harmonize assay methods and improve collaboration between laboratories and clinicians for better diagnosis and optimal care.