HPLC-UV Analysis and Antioxidant Potential of Phenolic Compounds from Endemic Shrub of Arid Environment Tamarix pauciovulata J. Gay

Abstract

This research presents complete phenolic compounds and biological activity of Tamarix pauciovulata J. Gay, an endemic Saharan species. The antioxidant assays revealed that crude extract showed strong DPPH scavenging activity (IC50 = 49.357 μg/mL) but in reducing power and hydrogen peroxide scavenging activity, butanolic and ethyl acetate fractions have a potent ferrous ion-chelating ability in particularly the butanolic fraction (63.18% reduced power at 50 μg/mL) and a powerful scavenging activity on hydrogen peroxide in particularly ethyl acetate fraction (IC50 = 0.205 μg/mL). The phenolic compounds of Tamarix pauciovulata leaves were analyzed by HPLC-UV. The major phenolic of leaf extracts are syringic acid (1.07 g/100g), quercetin (34.1 mg/100g), kaempferol (5.77 mg/100g), isorhamnetin (5 mg/100g). Others phenols were identified such as isoquercetin, catechin, epicatechin and its derivatives. Results indicated that the leaves extracts from Tamarix pauciovulata have great capacities to prevent diseases caused by the overproduction of radicals and can become important source of dietary compounds with health protective potential.